The Transcription Factor ASCIZ and Its Target DYNLL1 Are Essential for the Development and Expansion of MYC-Driven B Cell Lymphoma

Cell Rep. 2016 Feb 16;14(6):1488-1499. doi: 10.1016/j.celrep.2016.01.012. Epub 2016 Jan 28.

Abstract

How MYC promotes the development of cancer remains to be fully understood. Here, we report that the Zn(2+)-finger transcription factor ASCIZ (ATMIN, ZNF822) synergizes with MYC to activate the expression of dynein light chain (DYNLL1, LC8) in the murine Eμ-Myc model of lymphoma. Deletion of Asciz or Dynll1 prevented the abnormal expansion of pre-B cells in pre-cancerous Eμ-Myc mice and potentiated the pro-apoptotic activity of MYC in pre-leukemic immature B cells. Constitutive loss of Asciz or Dynll1 delayed lymphoma development in Eμ-Myc mice, and induced deletion of Asciz in established lymphomas extended the survival of tumor-bearing mice. We propose that ASCIZ-dependent upregulation of DYNLL1 levels is essential for the development and expansion of MYC-driven lymphomas by enabling the survival of pre-neoplastic and malignant cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology
  • Cell Cycle / genetics
  • Cell Differentiation
  • Cell Proliferation
  • Cytoplasmic Dyneins
  • Disease Models, Animal
  • Dyneins / deficiency
  • Dyneins / genetics*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / immunology
  • Lymphoma, B-Cell / mortality
  • Lymphoma, B-Cell / pathology
  • Mice
  • Precursor Cells, B-Lymphoid / immunology
  • Precursor Cells, B-Lymphoid / pathology*
  • Proto-Oncogene Proteins c-myc / genetics*
  • Proto-Oncogene Proteins c-myc / immunology
  • Signal Transduction
  • Survival Analysis
  • Transcription Factors / deficiency
  • Transcription Factors / genetics*

Substances

  • ATMIN protein, mouse
  • Myc protein, mouse
  • Proto-Oncogene Proteins c-myc
  • Transcription Factors
  • Cytoplasmic Dyneins
  • DYNLL1 protein, mouse
  • Dyneins