Enrichment of Mammary Basal and Luminal Cells for Cell-of-Origin Metastasis Studies

Farhia Kabeer; Katrina Podsypanina; Guillaume Darrasse-Jèze

doi:10.1101/pdb.prot078345

Enrichment of Mammary Basal and Luminal Cells for Cell-of-Origin Metastasis Studies

Cold Spring Harb Protoc. 2016 Feb 1;2016(2):pdb.prot078345. doi: 10.1101/pdb.prot078345.

Authors

Farhia Kabeer¹, Katrina Podsypanina², Guillaume Darrasse-Jèze³

Affiliations

¹ Institut de Recherches Cliniques de Montréal, Montréal, Quebec H2W 1R7, Canada; Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 1A3, Canada;
² Institut de Recherches Cliniques de Montréal, Montréal, Quebec H2W 1R7, Canada; Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 1A3, Canada; Pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Quebec H3T 1J4, Canada;
³ Université Pierre et Marie Curie, Université Paris 06; CNRS, Unité Mixte de Recherche 7211; INSERM, Unité Mixte de Recherche S959, I3, F-75013 Paris, France; University Paris Descartes, Sorbonne Paris Cité, Hôpital Necker-Enfants Malades, Faculté de Médecine Paris Descartes; INSERM, Unité 1013, F-75015 Paris, France; Laboratory of Molecular Immunology, Rockefeller University, New York, New York 10065.

PMID: 26832681
DOI: 10.1101/pdb.prot078345

Abstract

The mammary gland is an important model system in metastasis research. Mammary epithelial stem cells are of particular interest because of their capacity for regeneration and their role in cancer initiation. This protocol describes how to enrich for mammary basal and luminal epithelial cells using fluorescence-activated cell sorting (FACS).

MeSH terms

Animals
Epithelial Cells / physiology*
Flow Cytometry / methods*
Mammary Neoplasms, Animal / pathology*
Mice
Neoplasm Metastasis / pathology*
Neoplasm Metastasis / physiopathology*