ADPr-ChAP: Mapping ADP-Ribosylation onto the Genome

Robert Lyle McPherson; Anthony K L Leung

doi:10.1016/j.molcel.2016.01.023

ADPr-ChAP: Mapping ADP-Ribosylation onto the Genome

Mol Cell. 2016 Feb 4;61(3):327-328. doi: 10.1016/j.molcel.2016.01.023.

Authors

Robert Lyle McPherson¹, Anthony K L Leung²

Affiliations

¹ Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
² Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: [email protected].

PMID: 26849191
DOI: 10.1016/j.molcel.2016.01.023

Abstract

In this issue of Molecular Cell, Bartolomei et al. (2016) describe a chromatin affinity precipitation method using well-characterized ADP-ribose binding domains to provide the first genome-wide view of ADP-ribosylated chromatin. Here, we discuss its potential applications and the remaining challenges ahead.

Publication types

Comment

MeSH terms

Adenosine Diphosphate Ribose / metabolism*
Adipocytes / metabolism*
Animals
Cell Nucleus / metabolism*
Chromatin / metabolism*
Chromatin Immunoprecipitation / methods*
Humans

Substances

Chromatin
Adenosine Diphosphate Ribose

Grants and funding

R01 GM104135/GM/NIGMS NIH HHS/United States