Gene-expression Classifier in Papillary Thyroid Carcinoma: Validation and Application of a Classifier for Prognostication

Anticancer Res. 2016 Feb;36(2):749-56.

Abstract

Background: No reliable biomarker for metastatic potential in the risk stratification of papillary thyroid carcinoma exists. We aimed to develop a gene-expression classifier for metastatic potential.

Materials and methods: Genome-wide expression analyses were used. Development cohort: freshly frozen tissue from 38 patients was collected between the years 1986 and 2009. Validation cohort: formalin-fixed paraffin-embedded tissues were collected from 183 consecutively treated patients.

Results: A 17-gene classifier was identified based on the expression values in patients with and without metastasis in the development cohort. The 17-gene classifier for regional/distant metastasis identified was tested against the clinical status in the validation cohort. Sensitivity for detection of metastases was 51.5% and specificity 61.6%. Log-rank testing failed to identify any significance (p=0.32) regarding the classifier's usefulness as a prognostic marker for recurrence.

Conclusion: A 17-gene classifier for metastatic potential was developed, and the results showed a clear biological difference between groups. However, through validation, no prognostic significance of this classifier was shown.

Keywords: 749; Thyroid cancer; gene expression; metastasis; papillary thyroid carcinoma; prognostic indicators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Biomarkers, Tumor / genetics*
  • Carcinoma / classification
  • Carcinoma / genetics*
  • Carcinoma / mortality
  • Carcinoma / secondary
  • Carcinoma / surgery
  • Carcinoma, Papillary
  • Female
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation, Neoplastic
  • Genome-Wide Association Study
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Neoplasm Recurrence, Local
  • Predictive Value of Tests
  • Reproducibility of Results
  • Risk Assessment
  • Risk Factors
  • Thyroid Cancer, Papillary
  • Thyroid Neoplasms / classification
  • Thyroid Neoplasms / genetics*
  • Thyroid Neoplasms / mortality
  • Thyroid Neoplasms / pathology
  • Thyroid Neoplasms / surgery
  • Thyroidectomy
  • Treatment Outcome

Substances

  • Biomarkers, Tumor