Preclinical Experimental and Mathematical Approaches for Assessing Effective Doses of Inhaled Drugs, Using Mometasone to Support Human Dose Predictions

J Aerosol Med Pulm Drug Deliv. 2016 Aug;29(4):362-77. doi: 10.1089/jamp.2015.1253. Epub 2016 Feb 9.

Abstract

Background: Understanding the relationship between dose, lung exposure, and drug efficacy continues to be a challenging aspect of inhaled drug development. An experimental inhalation platform was developed using mometasone furoate to link rodent lung exposure to its in vivo pharmacodynamic (PD) effects.

Methods: We assessed the effect of mometasone delivered directly to the lung in two different rodent PD models of lung inflammation. The data obtained were used to develop and evaluate a mathematical model to estimate drug dissolution, transport, distribution, and efficacy, following inhaled delivery in rodents and humans.

Results: Mometasone directly delivered to the lung, in both LPS and Alternaria alternata rat models, resulted in dose dependent inhibition of BALf cellular inflammation. The parameters for our mathematical model were calibrated to describe the observed lung and systemic exposure profiles of mometasone in humans and in animal models. We found that physicochemical properties, such as lung fluid solubility and lipophilicity, strongly influenced compound distribution and lung retention.

Conclusions: Presently, we report on a novel and sophisticated mathematical model leading to improvements in a current inhaled drug development practices by providing a quantitative understanding of the relationship between PD effects and drug concentration in lungs.

Keywords: PK/PD modeling; drug dissolution and distribution; inhaled corticosteroid; inhaled delivery; inhaled drug development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Inhalation
  • Aerosols
  • Alternaria
  • Alternariosis / drug therapy*
  • Alternariosis / metabolism
  • Alternariosis / microbiology
  • Alternariosis / physiopathology
  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / pharmacokinetics
  • Disease Models, Animal
  • Drug Dosage Calculations*
  • Humans
  • Lipopolysaccharides
  • Lung / drug effects*
  • Lung / metabolism
  • Lung / physiopathology
  • Lung Diseases, Fungal / drug therapy*
  • Lung Diseases, Fungal / metabolism
  • Lung Diseases, Fungal / microbiology
  • Lung Diseases, Fungal / physiopathology
  • Male
  • Models, Biological*
  • Mometasone Furoate / administration & dosage*
  • Mometasone Furoate / pharmacokinetics
  • Pneumonia / chemically induced
  • Pneumonia / drug therapy*
  • Pneumonia / metabolism
  • Pneumonia / physiopathology
  • Rats, Inbred BN
  • Rats, Sprague-Dawley
  • Species Specificity
  • Tissue Distribution

Substances

  • Aerosols
  • Anti-Inflammatory Agents
  • Lipopolysaccharides
  • Mometasone Furoate