Effect of Schistosoma mansoni Infection on Innate and HIV-1-Specific T-Cell Immune Responses in HIV-1-Infected Ugandan Fisher Folk

AIDS Res Hum Retroviruses. 2016 Jul;32(7):668-75. doi: 10.1089/AID.2015.0274. Epub 2016 Mar 15.

Abstract

In Uganda, fisher folk have HIV prevalence rates, about four times higher than the national average, and are often coinfected with Schistosoma mansoni. We hypothesized that innate immune responses and HIV-specific Th1 immune responses might be downmodulated in HIV/S. mansoni-coinfected individuals compared with HIV+/S. mansoni-negative individuals. We stimulated whole blood with innate receptor agonists and analyzed supernatant cytokines by Luminex. We evaluated HIV-specific responses by intracellular cytokine staining for IFN-γ, IL-2, and TNF-α. We found that the plasma viral load and CD4 count were similar between the HIV+SM+ and HIV+SM- individuals. In addition, the TNF-α response to the imidazoquinoline compound CL097 and β-1, 3-glucan (curdlan), was significantly higher in HIV/S. mansoni-coinfected individuals compared with HIV only-infected individuals. The frequency of HIV-specific IFN-γ+IL-2-TNF-α- CD8 T cells and IFN-γ+IL-2-TNF-α+ CD4 T cells was significantly higher in HIV/S. mansoni-coinfected individuals compared with HIV only-infected individuals. These findings do not support the hypothesis that S. mansoni downmodulates innate or HIV-specific Th1 responses in HIV/S. mansoni-coinfected individuals.

MeSH terms

  • Adult
  • CD4 Lymphocyte Count
  • CD8-Positive T-Lymphocytes / immunology
  • Cytokines / biosynthesis
  • Female
  • HIV Infections / complications*
  • HIV Infections / immunology*
  • HIV-1 / immunology*
  • HIV-1 / isolation & purification
  • Humans
  • Immunity, Innate*
  • Male
  • Middle Aged
  • Schistosomiasis mansoni / complications*
  • Schistosomiasis mansoni / immunology*
  • Staining and Labeling
  • T-Lymphocytes / immunology*
  • Uganda
  • Viral Load
  • Young Adult

Substances

  • Cytokines