New crystal forms of the small subunit of ribonucleotide reductase from Escherichia coli

P Nordlund; U Uhlin; C Westergren; T Joelsen; B M Sjöberg; H Eklund

doi:10.1016/0014-5793(89)81666-7

New crystal forms of the small subunit of ribonucleotide reductase from Escherichia coli

FEBS Lett. 1989 Dec 4;258(2):251-4. doi: 10.1016/0014-5793(89)81666-7.

Authors

P Nordlund¹, U Uhlin, C Westergren, T Joelsen, B M Sjöberg, H Eklund

Affiliation

¹ Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.

PMID: 2689216
DOI: 10.1016/0014-5793(89)81666-7

Abstract

The small subunit of ribonucleotide reductase from Escherichia coli has been crystallized in two new crystal forms. The form most suitable for X-ray analysis belongs to the orthorhombic space group P2(1)2(1)2(1). It has the cell dimensions 74.3 A, 85.5 A, 115.7 A and diffracts to about 2.1 A resolution. The asymmetric unit most probably contains one dimer. Absorption spectra of single crystals confirm that the crystals contain a binuclear iron center. Crystals of the iron-depleted apoenzyme have also been obtained.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Escherichia coli / enzymology*
Kinetics
Protein Conformation
Ribonucleotide Reductases* / isolation & purification
Ribonucleotide Reductases* / metabolism
X-Ray Diffraction

Substances

Ribonucleotide Reductases