Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy

Clin Exp Allergy. 2016 May;46(5):764-74. doi: 10.1111/cea.12721.

Abstract

Background: Non-specific lipid transfer proteins (nsLTP) are considered to provoke allergic symptoms to plane tree pollen, which are frequently associated with peach allergy.

Objective: The objective was to clone the cDNA of plane pollen nsLTP Pla a 3, to characterize IgE-binding and allergenic potency of recombinant Pla a 3 in comparison to its natural counterpart and peach nsLTP Pru p 3.

Methods: Natural Pla a 3 was purified from plane pollen and analysed by mass spectrometry (MS). Recombinant Pla a 3 was characterized by SDS-PAGE and CD spectroscopy. Specific IgE to extract, components of plane pollen and Pru p 3 was measured by ImmunoCAP in sera of patients allergic to either plane pollen (n = 10), peach (n = 15) or both (n = 15). Biological potency of the proteins was investigated by in vitro mediator release assays and IgE cross-reactivity by competitive ELISA.

Results: Two Pla a 3 isoforms were identified. Recombinant Pla a 3 showed high purity, structural integrity, IgE-binding capacity comparable to nPla a 3 and biological potency. Sensitization to plane pollen extract was confirmed in 24/25 plane pollen allergics. The frequency of sensitization to Pla a 3 was 53% among patients allergic to both plane pollen and peach and 10% among plane pollen allergics tolerating peach where most patients were sensitized to Pla a 1. Pla a 3 and Pru p 3 showed strong bi-directional IgE cross-reactivity in patients allergic to peach and plane pollen, but not in peach allergics tolerating plane pollen. Levels of IgE-binding were generally higher to Pru p 3 than to Pla a 3.

Conclusion: Sensitization to Pla a 3 is relevant in a subgroup of plane pollen allergics with concomitant peach allergy. IgE testing with Pla a 3 may serve as a marker to identify plane pollen allergic patients at risk of LTP-mediated food reactions and thereby improve in vitro diagnostic procedures.

Keywords: LTP syndrome; Platanus allergy; Pla a 3; Pru p 3; peach allergy; plane pollen.

MeSH terms

  • Allergens / immunology*
  • Amino Acid Sequence
  • Antigens, Plant / chemistry
  • Antigens, Plant / genetics*
  • Antigens, Plant / immunology*
  • Biomarkers
  • Cloning, Molecular*
  • Cross Reactions / immunology
  • Food Hypersensitivity / diagnosis*
  • Food Hypersensitivity / immunology*
  • Gene Expression
  • Histamine Release
  • Humans
  • Immunization
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Phenotype
  • Pollen / immunology
  • Protein Isoforms
  • Prunus persica / adverse effects*
  • Recombinant Proteins

Substances

  • Allergens
  • Antigens, Plant
  • Biomarkers
  • Protein Isoforms
  • Recombinant Proteins
  • Immunoglobulin E

Associated data

  • GENBANK/AJ277163
  • GENBANK/AM286249
  • GENBANK/KR092033
  • GENBANK/KR139936