Direct visualization of an IgM directed against a membrane antigen involved in both cell-matrix interactions and microfilament organization

H Lesot; J M Meyer; A Staubli; D A Schmitt; J V Ruch

Direct visualization of an IgM directed against a membrane antigen involved in both cell-matrix interactions and microfilament organization

Biol Cell. 1989;66(3):335-8.

Authors

H Lesot¹, J M Meyer, A Staubli, D A Schmitt, J V Ruch

Affiliation

¹ Institut de Biologie Médicale, CNRS LP 6520, Faculté de Médecine 11, Strasbourg, France.

PMID: 2690988

Abstract

Dental mesenchymal cells were cultured in the presence of a monoclonal antibody. MC16A16, consisting of IgM and directed against a hydrophobic 165 kDa protein. Since the epitope recognized by MC16A16 was found to be at the outer cell surface, a direct visualization of IgM antibodies was used to localize the 165 kDa antigen by transmission electron microscopy. The present results demonstrate that the 165 kDa antigen is a membrane protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / ultrastructure
Animals
Antibodies, Monoclonal
Antigens, Surface / analysis*
Fluorescent Antibody Technique
Immunoglobulin M
Immunohistochemistry
Membrane Proteins / analysis
Mice
Microscopy, Electron
Molecular Weight
Octoxynol
Polyethylene Glycols
Tooth / cytology*

Substances

Antibodies, Monoclonal
Antigens, Surface
Immunoglobulin M
Membrane Proteins
Polyethylene Glycols
Octoxynol
Nonidet P-40