Abstract
Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Boric Acids / metabolism
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Cell-Free System / chemistry
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Cell-Free System / metabolism*
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Dielectric Spectroscopy
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Esters
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Hordeum
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Hydrogen-Ion Concentration
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Immunoblotting
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Lipid Bilayers / chemistry*
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Membrane Transport Proteins / biosynthesis*
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Membrane Transport Proteins / chemistry
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Phosphatidylcholines / chemistry
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Plant Proteins / biosynthesis*
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Plant Proteins / chemistry
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RNA, Messenger / genetics
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RNA, Plant / genetics
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Thioctic Acid / chemistry
Substances
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Boric Acids
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Esters
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Lipid Bilayers
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Membrane Transport Proteins
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Phosphatidylcholines
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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1,2-diphytanoylphosphatidylcholine
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Thioctic Acid
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boric acid