Comparative evaluation of whole blood versus plasma for quantitative detection of cytomegalovirus using an automated system

Sri Suganda; Li Tang; Jeanne Carr; Yilun Sun; Stanley Pounds; Randall Hayden

doi:10.1016/j.diagmicrobio.2015.12.008

Comparative evaluation of whole blood versus plasma for quantitative detection of cytomegalovirus using an automated system

Diagn Microbiol Infect Dis. 2016 May;85(1):23-5. doi: 10.1016/j.diagmicrobio.2015.12.008. Epub 2015 Dec 17.

Authors

Sri Suganda¹, Li Tang², Jeanne Carr¹, Yilun Sun², Stanley Pounds², Randall Hayden³

Affiliations

¹ Department of Pathology, St Jude Children's Research Hospital, Memphis, TN, USA.
² Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN, USA.
³ Department of Pathology, St Jude Children's Research Hospital, Memphis, TN, USA. Electronic address: [email protected].

PMID: 26935108
DOI: 10.1016/j.diagmicrobio.2015.12.008

Abstract

Peripheral blood samples were separated into aliquots of whole blood and plasma and tested using an automated PCR system. More positive results were obtained from plasma (43), compared to whole blood (34). Plasma results did not correlate well with those in whole blood and tended to produce higher viral loads.

Keywords: CMV; Cytomegalovirus; PCR; Quantitation; Sample type.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Automation, Laboratory
Cytomegalovirus / genetics*
Cytomegalovirus Infections / diagnosis*
Cytomegalovirus Infections / virology*
Humans
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Viral Load / methods*
Viral Load / standards