The effects of aminophylline (Ami) on immune systems in normal NIH mice were studied. Ami ig 25, 50 and 100 mg/(kg.d) x 8-10 d increased hemolytic ability of plaque forming cells and antibody concentration while it decreased the delayed type hypersensitivity (DTH) reaction and peripheral white blood cells phagocytosing function. Ami 1, 5, 10 micrograms/ml also promoted lymphocyte transformation of [methyl-3H] TdR incorporation induced by PHA in vitro.