Linear chromosome of Borrelia burgdorferi

C Baril; C Richaud; G Baranton; I S Saint Girons

doi:10.1016/0923-2508(89)90083-1

Linear chromosome of Borrelia burgdorferi

Res Microbiol. 1989 Oct;140(8):507-16. doi: 10.1016/0923-2508(89)90083-1.

Authors

C Baril¹, C Richaud, G Baranton, I S Saint Girons

Affiliation

¹ Unité des Leptospires, Institut Pasteur, Paris.

PMID: 2696058
DOI: 10.1016/0923-2508(89)90083-1

Abstract

The DNA organization of several European and American isolates of Borrelia burgdorferi, the aetiological agent of Lyme disease, was analysed in pulse-field agarose gel electrophoresis. The results of in situ cell lysis in agarose plugs demonstrated a unique arrangement for the DNA of this spirochete. The chromosome of Borrelia behaved as a eukaryotic linear chromosome with a size of around 1,000 kb. The genome also comprised several circular and linear plasmids which varied in size from 15 to 60 kb.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Borrelia burgdorferi Group / genetics*
DNA, Bacterial / isolation & purification*
Electrophoresis, Agar Gel
In Vitro Techniques
Molecular Weight
Nucleic Acid Hybridization
Plasmids / genetics
Restriction Mapping

Substances

DNA, Bacterial