Abstract
The partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with Achromobacter protease I. The results showed the amino-terminal sequence of the mature enzyme to be (N) Ser-Leu-Pro-Pro-His-Gln-Lys-Val-Pro-Leu-Pro-Ser- Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys. In addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases* / analysis
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Acetyltransferases* / metabolism
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Alcaligenes / enzymology
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Amino Acid Sequence
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Amino Acids / analysis
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Animals
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Binding Sites
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Cattle
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Chromatography, High Pressure Liquid
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Dihydrolipoyllysine-Residue Acetyltransferase
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Humans
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Molecular Sequence Data
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Molecular Weight
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Peptide Hydrolases / metabolism
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Pyruvate Dehydrogenase Complex*
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Rats
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Sequence Homology, Nucleic Acid
Substances
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Amino Acids
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Pyruvate Dehydrogenase Complex
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Acetyltransferases
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Dihydrolipoyllysine-Residue Acetyltransferase
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Peptide Hydrolases