Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells

Peggy Matz; James Adjaye

doi:10.1016/j.scr.2015.10.004

Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells

Stem Cell Res. 2015 Nov;15(3):581-3. doi: 10.1016/j.scr.2015.10.004. Epub 2015 Oct 14.

Authors

Peggy Matz¹, James Adjaye²

Affiliations

¹ Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute of Biology, Humboldt University, 10099 Berlin, Germany.
² Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.

PMID: 26987922
DOI: 10.1016/j.scr.2015.10.004

Abstract

Human umbilical vein endothelial cells (HUVECs) were used to generate the iPSC line epiHUVEC employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHUVEC and the human embryonic stem cell line - H1 have a Pearson correlation of 0.899.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line
Human Umbilical Vein Endothelial Cells / metabolism*
Humans
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4