Generation of iPSC lines from primary human chorionic villi cells

Björn Lichtner; Peggy Matz; James Adjaye

doi:10.1016/j.scr.2015.10.011

Generation of iPSC lines from primary human chorionic villi cells

Stem Cell Res. 2015 Nov;15(3):697-9. doi: 10.1016/j.scr.2015.10.011. Epub 2015 Oct 23.

Authors

Björn Lichtner¹, Peggy Matz², James Adjaye³

Affiliations

¹ Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany.
² Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
³ Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: [email protected].

PMID: 26987929
DOI: 10.1016/j.scr.2015.10.011

Abstract

Primary human chorionic villi (CV) cells were used to generate the iPSC line by retroviral transduction of the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC. Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines-H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques
Cell Differentiation
Cellular Reprogramming
Chorionic Villi / immunology*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4