Generation of iPSC lines from primary human amniotic fluid cells

Katharina Drews; Peggy Matz; James Adjaye

doi:10.1016/j.scr.2015.11.003

Generation of iPSC lines from primary human amniotic fluid cells

Stem Cell Res. 2015 Nov;15(3):712-4. doi: 10.1016/j.scr.2015.11.003. Epub 2015 Nov 11.

Authors

Katharina Drews¹, Peggy Matz², James Adjaye³

Affiliations

¹ Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany.
² Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
³ Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany; Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: [email protected].

PMID: 26987930
DOI: 10.1016/j.scr.2015.11.003

Abstract

By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amniotic Fluid / metabolism*
Cell Differentiation
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Proto-Oncogene Proteins c-myc / metabolism*

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Proto-Oncogene Proteins c-myc