Abstract
A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / antagonists & inhibitors
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Bacterial Proteins / classification*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Boronic Acids / pharmacology
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Cloxacillin / pharmacology
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Edetic Acid / pharmacology
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Enterobacteriaceae / drug effects*
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Enterobacteriaceae / enzymology
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Enterobacteriaceae / genetics
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Enterobacteriaceae / growth & development
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Enterobacteriaceae Infections / microbiology
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Enzyme Inhibitors / pharmacology*
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Flow Cytometry / methods*
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Gene Expression
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Humans
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Meropenem
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Penicillins / pharmacology
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Thienamycins / pharmacology*
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beta-Lactamases / classification*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Boronic Acids
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Enzyme Inhibitors
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Penicillins
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Thienamycins
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temocillin
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3-aminobenzeneboronic acid
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Edetic Acid
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beta-Lactamases
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carbapenemase
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Meropenem
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Cloxacillin