Species-specific real-time PCR assay for the detection of Streptococcus suis from clinical specimens

Velusamy Srinivasan; Lesley McGee; Berthe-Marie Njanpop-Lafourcade; Jennifer Moïsi; Bernard Beall

doi:10.1016/j.diagmicrobio.2016.02.013

Species-specific real-time PCR assay for the detection of Streptococcus suis from clinical specimens

Diagn Microbiol Infect Dis. 2016 Jun;85(2):131-2. doi: 10.1016/j.diagmicrobio.2016.02.013. Epub 2016 Feb 18.

Authors

Velusamy Srinivasan¹, Lesley McGee², Berthe-Marie Njanpop-Lafourcade³, Jennifer Moïsi³, Bernard Beall²

Affiliations

¹ Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: [email protected].
² Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
³ Agence de Médecine Préventive, Paris, France.

PMID: 27041105
DOI: 10.1016/j.diagmicrobio.2016.02.013

Abstract

A real-time polymerase chain reaction was developed to detect all known strains of Streptococcus suis. The assay was highly specific, and sensitivity was <10 copies/assay for S. suis detection from clinical samples.

Keywords: Clinical specimens; Detection; Real-time PCR; S. suis.

Published by Elsevier Inc.

MeSH terms

Animals
Humans
Molecular Diagnostic Techniques / methods*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Streptococcal Infections / diagnosis*
Streptococcal Infections / veterinary*
Streptococcus suis / genetics
Streptococcus suis / isolation & purification*
Swine