Loss of IL-22 inhibits autoantibody formation in collagen-induced arthritis in mice

Eur J Immunol. 2016 Jun;46(6):1404-14. doi: 10.1002/eji.201546241. Epub 2016 May 12.

Abstract

Interleukin 22 (IL-22) expression is associated with increased joint destruction and disease progression in rheumatoid arthritis (RA). Although IL-22 is considered a pro-inflammatory cytokine, its mechanism of action in RA remains incompletely understood. Here, we used the collagen-induced arthritis model in IL-22 deficient (IL-22(-/-) ) mice to study the role of IL-22 in RA. In spite of normal disease incidence, disease severity is significantly diminished in IL-22(-/-) mice. Moreover, pathogenicity of Th17 cells and development and function of B cells are unaffected. In contrast, splenic plasma cells, as well as serum autoantibody titers, are reduced in the absence of IL-22. At the peak of disease, germinal centers (GCs) are severely reduced in the spleens of IL-22(-/-) mice, correlating with a decline in GC B-cell numbers. Within the GC, we identified IL-22R1 expressing follicular dendritic cell-like stromal cells. Human lymphoid stromal cells respond to IL-22 ex vivo by inducing transcription of CXCL12 and CXCL13. We therefore postulate IL-22 as an important enhancer of the GC reaction, maintaining chemokine levels for the persistence of GC reactions, essential for the production of autoantibody-secreting plasma cells. Blocking IL-22 might therefore prevent immune-complex deposition and destruction of joints in RA patients.

Keywords: Autoantibody formation; B cell; Germinal center; Interleukin 22 (IL-22); Rheumatoid arthritis.

MeSH terms

  • Animals
  • Antibody Formation / genetics*
  • Antibody Formation / immunology*
  • Antibody Specificity / immunology
  • Arthritis, Experimental / etiology*
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology
  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / metabolism
  • Arthritis, Rheumatoid / pathology
  • Autoantibodies / immunology*
  • Cells, Cultured
  • Chemokines / genetics
  • Chemokines / metabolism
  • Coculture Techniques
  • Disease Models, Animal
  • Germinal Center / immunology
  • Germinal Center / metabolism
  • Interleukin-22
  • Interleukins / deficiency*
  • Lymphocyte Activation
  • Mice
  • Mice, Knockout
  • Plasma Cells / immunology
  • Plasma Cells / metabolism
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / metabolism
  • Severity of Illness Index
  • Stromal Cells / metabolism
  • Th17 Cells / immunology
  • Th17 Cells / metabolism

Substances

  • Autoantibodies
  • Chemokines
  • Interleukins
  • Receptors, Interleukin
  • interleukin-22 receptor