Alteration in the concentrations of Interleukin-7 (IL-7), Interleukin-10 (IL-10) and Granulocyte Colony Stimulating Factor (G-CSF) in alcohol-dependent individuals without liver disease, during detoxification therapy

Drug Alcohol Depend. 2016 Jun 1:163:77-83. doi: 10.1016/j.drugalcdep.2016.03.022. Epub 2016 Mar 31.

Abstract

Background: The course of Interleukin-7 (IL-7), Interleukin-10 (IL-10) and Granulocyte Colony Stimulating Factor (G-CSF) was investigated in alcohol-dependent individuals without liver disease in order to ascertain the use of these cytokines as markers for the follow-up testing and the outcome of the detoxification treatment.

Methods: Forty-eight alcohol-dependent individuals were admitted for alcohol detoxification. Blood was obtained upon admission, two weeks later and after the completion of the detoxification period (4-5 weeks). Serum IL-7, IL-10 and G-CSF were measured with a commercially available sandwich enzyme immunoassay.

Results: IL-7 concentration was steadily high from admission up to two weeks later and then showed a fall, yet still remaining significantly higher than in the control group at the end of the detoxification treatment. IL-10 concentration was significantly low on admission, presenting a linear increase during therapy and remained insignificantly low at the end. G-CSF was significantly elevated on admission and presented a linear fall ending up in almost normal values at the end of the detoxification therapy.

Conclusions: The alterations in the concentration of IL-7, IL-10 and G-CSF and their trend to normalization during the detoxification therapy are indicative of the generalized immune system disorder, caused by alcohol abuse. Further studies will help in further elucidating the pathophysiology of the immune system function in alcohol abuse, while immunological parameters might serve as biological markers and diagnostic tools for the assessment of the course and the outcome of the detoxification therapy.

Keywords: Alcohol abuse; Detoxification therapy; Granulocyte Colony Stimulating Factor; Interleukin-10; Interleukin-7; Liver disease.

MeSH terms

  • Adult
  • Aged
  • Alcoholism / blood*
  • Alcoholism / diagnosis
  • Alcoholism / therapy*
  • Biomarkers / blood
  • Cytokines / blood
  • Female
  • Granulocyte Colony-Stimulating Factor / blood*
  • Humans
  • Interleukin-10 / blood*
  • Interleukin-7 / blood*
  • Liver Diseases
  • Male
  • Middle Aged
  • Patient Admission / trends
  • Substance Abuse Treatment Centers*
  • Young Adult

Substances

  • Biomarkers
  • Cytokines
  • IL10 protein, human
  • IL7 protein, human
  • Interleukin-7
  • Interleukin-10
  • Granulocyte Colony-Stimulating Factor