Comparison of Matrix-Based and Filter Paper-Based Systems for Transport of Plasma for HIV-1 RNA Quantification and Amplicon Preparation for Genotyping

J Clin Microbiol. 2016 Jul;54(7):1899-1901. doi: 10.1128/JCM.00541-16. Epub 2016 Apr 13.

Abstract

Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensitivity of genotyping specimens with HIV-1 RNA of <4.70 log10 copies/ml.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Genotyping Techniques / methods*
  • HIV Infections / virology*
  • HIV-1 / classification*
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Plasma / virology
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification*
  • Sensitivity and Specificity
  • Specimen Handling / methods*
  • Viral Load / methods*

Substances

  • RNA, Viral