Abstract
AlbA is a radical SAM enzyme catalyzing the formation of three unusual thioether bonds in the antibiotic subtilosin A. We demonstrate here that AlbA catalyzes direct Cα H-atom abstraction and likely contains three essential [4Fe-4S] centers. This leads us to propose novel mechanistic perspectives for thioether bond catalysis by radical SAM enzymes.
MeSH terms
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Anti-Bacterial Agents / biosynthesis*
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Anti-Bacterial Agents / chemistry
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Bacteriocins / biosynthesis*
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Bacteriocins / chemistry
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Biocatalysis
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Free Radicals / chemistry
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Free Radicals / metabolism
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Models, Molecular
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Peptides, Cyclic / biosynthesis*
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Peptides, Cyclic / chemistry
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Sulfides / chemistry
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Sulfides / metabolism*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Bacteriocins
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Free Radicals
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Peptides, Cyclic
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Sulfides
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sboA protein, Bacillus subtilis