Mechanisms of mitophagy: PINK1, Parkin, USP30 and beyond

Free Radic Biol Med. 2016 Nov:100:210-222. doi: 10.1016/j.freeradbiomed.2016.04.015. Epub 2016 Apr 16.

Abstract

Mitochondrial quality control is central for maintaining a healthy population of mitochondria. Two Parkinson's disease genes, mitochondrial kinase PINK1 and ubiquitin ligase Parkin, degrade damaged mitochondria though mitophagy. In this pathway, PINK1 senses mitochondrial damage and activates Parkin by phosphorylating Parkin and ubiquitin. Activated Parkin then builds ubiquitin chains on damaged mitochondria to tag them for degradation in lysosomes. USP30 deubiquitinase acts as a brake on mitophagy by opposing Parkin-mediated ubiquitination. Human genetic data point to a role for mitophagy defects in neurodegenerative diseases. This review highlights the molecular mechanisms of the mitophagy pathway and the recent advances in the understanding of mitophagy in vivo.

Keywords: Mitophagy; PINK1; Parkin; Parkinson's Disease; USP30.

Publication types

  • Review

MeSH terms

  • Humans
  • Mitochondrial Proteins / metabolism*
  • Mitochondrial Proteins / physiology
  • Mitophagy*
  • Parkinson Disease / metabolism
  • Parkinson Disease / physiopathology
  • Protein Kinases / metabolism*
  • Protein Kinases / physiology
  • Thiolester Hydrolases / metabolism*
  • Thiolester Hydrolases / physiology
  • Ubiquitin-Protein Ligases / metabolism*
  • Ubiquitin-Protein Ligases / physiology

Substances

  • Mitochondrial Proteins
  • Usp30 protein, human
  • Ubiquitin-Protein Ligases
  • parkin protein
  • Protein Kinases
  • PTEN-induced putative kinase
  • Thiolester Hydrolases