Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection

Simon P Preston; Marc Pellegrini; Gregor Ebert

doi:10.1007/978-1-4939-3581-9_9

Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection

Methods Mol Biol. 2016:1419:109-15. doi: 10.1007/978-1-4939-3581-9_9.

Authors

Simon P Preston^{1

2}, Marc Pellegrini^{1

2}, Gregor Ebert^{3

4}

Affiliations

¹ The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
² Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
³ The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia. [email protected].
⁴ Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia. [email protected].

PMID: 27108435
DOI: 10.1007/978-1-4939-3581-9_9

Abstract

Gene delivery methods are important for both therapeutic intervention and as tools in research to address specific questions. Hydrodynamic injection (HDI) is a method that facilitates the delivery and expression of genetic material in target cells, namely hepatocytes, through an intravenous injection. HDI has great utility for research involving cell death and signaling pathways essential in the processes of cancer, inflammation, and transplant therapy, as well as representing a valuable technique to establish hepatitis B virus (HBV) expression in hepatocytes. This chapter describes in detail how to generate a model of chronic HBV infection in immunocompetent mice using HDI as a delivery method.

Keywords: Gene delivery; HBV; Hydrodynamic injection; In vivo transfection.

MeSH terms

Animals
Disease Models, Animal*
Gene Transfer Techniques*
Genome, Viral
Hepatitis B virus / pathogenicity*
Hepatitis B, Chronic / virology*
Hydrodynamics*
Injections / methods*
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Plasmids*