Abstract
The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, N.I.H., Extramural
MeSH terms
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Binding Sites
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Cross-Linking Reagents / chemistry
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DNA, Complementary / genetics
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HeLa Cells
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High-Throughput Nucleotide Sequencing
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Humans
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Immunoprecipitation / methods*
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Photochemical Processes
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RNA-Binding Proteins / analysis*
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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RNA-Binding Proteins / radiation effects
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Sensitivity and Specificity
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Transcriptome
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Ultraviolet Rays*
Substances
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Cross-Linking Reagents
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DNA, Complementary
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RNA-Binding Proteins