Bothrops erythromelas () venom induces apoptosis on renal tubular epithelial cells

Toxicon. 2016 Aug:118:82-5. doi: 10.1016/j.toxicon.2016.04.040. Epub 2016 Apr 23.

Abstract

Bothrops erythromelas is responsible for a large number of snakebite incidents in Northeastern Brazil. Previously, we showed the effects of whole B. erythromelas venom in an isolated kidney model. To continue the study with B. erythromelas venom, the present work aims to study the effects of this venom on MDCK tubular epithelial cells and assess gene expression involved in kidney injury, aiming at elucidating the mechanisms responsible for renal toxicity. Cytotoxicity in MDCK cells showed an IC50 of 93 μg/mL and predominant apoptotic involvement demonstrated by flow cytometry assays and expression of caspase-3 and caspase-8. In conclusion, we suggest that Bothropoides erythromelas venom causes apoptosis with involvement of the caspases, probably through the extrinsic pathway.

Keywords: Apoptosis; Bothropoides erythromelas venom; MDCK cells.

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Bothrops*
  • Brazil
  • Caspase 3 / chemistry
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Caspase 8 / chemistry
  • Caspase 8 / genetics
  • Caspase 8 / metabolism
  • Cell Survival / drug effects
  • Crotalid Venoms / enzymology
  • Crotalid Venoms / toxicity*
  • Dogs
  • Gene Expression Regulation / drug effects*
  • Inhibitory Concentration 50
  • Kidney Tubules / drug effects*
  • Kidney Tubules / metabolism
  • Madin Darby Canine Kidney Cells
  • Metalloproteases / toxicity
  • RNA, Messenger / metabolism
  • Reptilian Proteins / agonists*
  • Reptilian Proteins / chemistry
  • Reptilian Proteins / metabolism
  • Reptilian Proteins / toxicity

Substances

  • Crotalid Venoms
  • RNA, Messenger
  • Reptilian Proteins
  • Metalloproteases
  • Caspase 3
  • Caspase 8