Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity

Science. 2016 Apr 29;352(6285):565-9. doi: 10.1126/science.aad3369. Epub 2016 Apr 28.

Abstract

Deep sequencing of the gut microbiomes of 1135 participants from a Dutch population-based cohort shows relations between the microbiome and 126 exogenous and intrinsic host factors, including 31 intrinsic factors, 12 diseases, 19 drug groups, 4 smoking categories, and 60 dietary factors. These factors collectively explain 18.7% of the variation seen in the interindividual distance of microbial composition. We could associate 110 factors to 125 species and observed that fecal chromogranin A (CgA), a protein secreted by enteroendocrine cells, was exclusively associated with 61 microbial species whose abundance collectively accounted for 53% of microbial composition. Low CgA concentrations were seen in individuals with a more diverse microbiome. These results are an important step toward a better understanding of environment-diet-microbe-host interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Chromogranin A / analysis
  • Chromogranin A / metabolism
  • Diet
  • Enteroendocrine Cells / metabolism
  • Feces / chemistry
  • Feces / microbiology
  • Gastrointestinal Microbiome / genetics*
  • Gastrointestinal Tract / microbiology*
  • Genetic Markers
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Metagenomics
  • Netherlands
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics

Substances

  • Chromogranin A
  • Genetic Markers
  • RNA, Ribosomal, 16S