A "turn-on" fluorescent chemosensor for the detection of Zn(II) in aqueous solution at neutral pH and its application in live cells imaging

Talanta. 2016 Jun 1:153:381-5. doi: 10.1016/j.talanta.2016.03.040. Epub 2016 Mar 13.

Abstract

Pyridoxal-2-hydrazinopyridine Schiff-base (1) was prepared by one-step synthesis from commercially available materials of pyridoxal and 2-hydrazinopyridine. Compound 1 could be applied as a fluorescent chemosensor for Zn(II) in aqueous solution at pH 7.4. After binding to Zn(II) with a molar ratio of 1:1, 1 exhibited 17-fold fluorescence enhancement. In addition, 1 showed a good selectivity toward Zn(II) over other metal ions, which can especially distinguish Zn(II) from Cd(II). A linear range of 5.0-20.0μmolL(-1) and a detection limit of 70nmolL(-1) were also achieved. Moreover, the utility of chemosensor 1 in bioimaging of Zn(II) in living cells was demonstrated, which suggested that 1 could be used as a Zn(II)-selective chemosensor in biological samples.

Keywords: Aqueous solution; Cell imaging; Fluorescent chemosensor; Zinc; “Turn on” response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival
  • Fluorescent Dyes
  • Hydrogen-Ion Concentration
  • Pyridones
  • Solutions
  • Spectrometry, Fluorescence*
  • Zinc

Substances

  • Fluorescent Dyes
  • Pyridones
  • Solutions
  • 2-hydrazinopyridine
  • Zinc