Remnant Lipoprotein Cholesterol and Incident Coronary Heart Disease: The Jackson Heart and Framingham Offspring Cohort Studies

J Am Heart Assoc. 2016 Apr 29;5(5):e002765. doi: 10.1161/JAHA.115.002765.

Abstract

Background: Remnant lipoproteins (RLPs), the triglyceride-enriched precursors to low-density lipoprotein, are an emerging risk factor for coronary heart disease (CHD). We sought to determine the association of RLP cholesterol (RLP-C) levels with incident CHD in 2 diverse, prospective, longitudinal observational US cohorts.

Methods and results: We analyzed cholesterol levels from serum lipoprotein samples separated via density gradient ultracentrifugation in 4114 US black participants (mean age 53.8 years, 64% women) from the Jackson Heart Study and a random sample of 818 predominantly white participants (mean age 57.3 years, 52% women) from the Framingham Offspring Cohort Study. Multivariable-adjusted hazard ratios (HRs) for RLP-C (the sum of very low-density lipoprotein3 cholesterol and intermediate-density lipoprotein cholesterol) were derived to estimate associations with incident CHD events consisting of myocardial infarction, CHD death, and revascularizations for each cohort separately and as a combined population. There were 146 CHD events in the combined population. After adjustments for age, sex, body mass index, smoking, blood pressure, diabetes, and lipid-lowering therapy for the combined population, RLP-C (HR 1.23 per 1-SD increase, 95% CI 1.06-1.42, P<0.01) and intermediate-density lipoprotein cholesterol (HR 1.26 per 1-SD increase, 95% CI 1.08-1.47, P<0.01) predicted CHD during an 8-year follow-up. Associations were attenuated by high-density lipoprotein cholesterol and ultimately lost significance with inclusion of real low-density lipoprotein cholesterol, which excludes Lp(a) and IDL cholesterol fractions. Similar associations were seen in multivariable analyses within each cohort.

Conclusion: RLP-C levels are predictive of incident CHD in this diverse group of primary prevention subjects. Interventions aimed at reducing RLP-C to prevent CHD warrant further intensive investigation.

Clinical trial registration: URL: http://www.ClinicalTrials.gov. Unique identifier: NCT00415415.

Keywords: coronary heart disease; lipids; primary prevention; remnant lipoprotein cholesterol; triglycerides.

Publication types

  • Observational Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cholesterol / blood*
  • Cholesterol, HDL / blood
  • Cholesterol, VLDL / blood*
  • Cohort Studies
  • Coronary Disease / blood
  • Coronary Disease / epidemiology*
  • Coronary Disease / mortality
  • Female
  • Humans
  • Incidence
  • Lipoprotein(a) / blood
  • Lipoproteins / blood*
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Myocardial Infarction / blood
  • Myocardial Infarction / epidemiology*
  • Myocardial Revascularization / statistics & numerical data*
  • Proportional Hazards Models
  • Prospective Studies
  • Risk Factors
  • Triglycerides / blood*
  • United States / epidemiology

Substances

  • Cholesterol, HDL
  • Cholesterol, VLDL
  • Lipoprotein(a)
  • Lipoproteins
  • Triglycerides
  • lipoprotein cholesterol
  • remnant-like particle cholesterol
  • Cholesterol

Associated data

  • ClinicalTrials.gov/NCT00415415