Ethanol increases the sensitivity of carnitine palmitoyltransferase I to inhibition by malonyl-CoA in short-term hepatocyte incubations

Biochim Biophys Acta. 1989 Apr 26;1002(3):405-8. doi: 10.1016/0005-2760(89)90358-5.

Abstract

The sensitivity of carnitine palmitoyltransferase I to inhibition by malonyl-CoA was increased in mitochondria isolated from rat hepatocytes incubated with ethanol. This effect was mimicked by incubation of hepatocytes with acetaldehyde or by preincubation of isolated mitochondria with malonyl-CoA. Both ethanol and acetaldehyde increased the intracellular concentration of malonyl-CoA. Results suggest that the ethanol-induced elevation of intracellular malonyl-CoA levels may be responsible for the enhanced sensitivity of carnitine palmitoyltransferase I to inhibition by malonyl-CoA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetaldehyde / pharmacology
  • Acyl Coenzyme A / pharmacology*
  • Acyltransferases / antagonists & inhibitors*
  • Animals
  • Carnitine O-Palmitoyltransferase / antagonists & inhibitors*
  • Cells, Cultured
  • Ethanol / pharmacology*
  • Malonyl Coenzyme A / pharmacology*
  • Mitochondria, Liver / enzymology*
  • Rats

Substances

  • Acyl Coenzyme A
  • Ethanol
  • Malonyl Coenzyme A
  • Acyltransferases
  • Carnitine O-Palmitoyltransferase
  • Acetaldehyde