Selective expression of mutant huntingtin during development recapitulates characteristic features of Huntington's disease

Proc Natl Acad Sci U S A. 2016 May 17;113(20):5736-41. doi: 10.1073/pnas.1603871113. Epub 2016 May 2.

Abstract

Recent studies have identified impairments in neural induction and in striatal and cortical neurogenesis in Huntington's disease (HD) knock-in mouse models and associated embryonic stem cell lines. However, the potential role of these developmental alterations for HD pathogenesis and progression is currently unknown. To address this issue, we used BACHD:CAG-Cre(ERT2) mice, which carry mutant huntingtin (mHtt) modified to harbor a floxed exon 1 containing the pathogenic polyglutamine expansion (Q97). Upon tamoxifen administration at postnatal day 21, the floxed mHtt-exon1 was removed and mHtt expression was terminated (Q97(CRE)). These conditional mice displayed similar profiles of impairments to those mice expressing mHtt throughout life: (i) striatal neurodegeneration, (ii) early vulnerability to NMDA-mediated excitotoxicity, (iii) impairments in motor coordination, (iv) temporally distinct abnormalities in striatal electrophysiological activity, and (v) altered corticostriatal functional connectivity and plasticity. These findings strongly suggest that developmental aberrations may play important roles in HD pathogenesis and progression.

Keywords: neurodegeneration; plasticity; prodromal.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Animals
  • Apoptosis
  • Corpus Striatum / pathology
  • Corpus Striatum / physiopathology
  • Female
  • GABAergic Neurons / physiology
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Humans
  • Huntingtin Protein / genetics*
  • Huntingtin Protein / metabolism
  • Huntington Disease / genetics*
  • Huntington Disease / metabolism
  • Huntington Disease / physiopathology
  • Male
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Muscle Strength
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Mutation, Missense
  • Organ Specificity
  • Rotarod Performance Test

Substances

  • Htt protein, mouse
  • Huntingtin Protein
  • Mutant Proteins