Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues

Naomi McGovern; Andreas Schlitzer; Baptiste Janela; Florent Ginhoux

doi:10.1007/978-1-4939-3606-9_12

Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues

Methods Mol Biol. 2016:1423:169-80. doi: 10.1007/978-1-4939-3606-9_12.

Authors

Naomi McGovern¹, Andreas Schlitzer¹, Baptiste Janela¹, Florent Ginhoux²

Affiliations

¹ Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.
² Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore. [email protected].

PMID: 27142016
DOI: 10.1007/978-1-4939-3606-9_12

Abstract

The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.

Keywords: Antigen-presenting cells; Dendritic cells; Flow cytometry; Human tissue processing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigen Presentation
Antigen-Presenting Cells / cytology*
Antigen-Presenting Cells / immunology
Antigens, CD1 / metabolism
Antigens, Surface / metabolism
Cell Separation / methods*
Dendritic Cells / cytology*
Dendritic Cells / immunology
Dermis / cytology
Dermis / immunology
Flow Cytometry / methods*
Humans
Kidney / cytology
Kidney / immunology
Liver / cytology
Liver / immunology
Lung / cytology
Lung / immunology
Mice

Substances

Antigens, CD1
Antigens, Surface