Molecular Biomarker Study in a Randomised Phase III Trial of Irinotecan Plus S-1 versus S-1 for Advanced Gastric Cancer (GC0301/TOP-002)

Clin Oncol (R Coll Radiol). 2016 Aug;28(8):e45-51. doi: 10.1016/j.clon.2016.04.001. Epub 2016 Apr 30.

Abstract

Aims: Gastric cancer is a common and heterogeneous disease; however, global standard and biomarkers for selecting chemotherapy regimens have not been established. This study was designed retrospectively to identify molecular biomarkers for irinotecan plus S-1 (IRI-S) and S-1 therapy from subset analyses in GC0301/TOP-002, a randomised phase III trial for advanced gastric cancer.

Materials and methods: Paraffin-embedded primary tumour specimens were collected from 126 of 326 randomised patients in GC0301/TOP-002. The mRNA was measured for thymidylate synthase, dihydropyrimidine dehydrogenase, topoisomerase I, excision repair cross-complementing gene 1 (ERCC1) and thymidine phosphorylase; categorised into low and high to analyse their association with efficacy end points.

Results: There was no significant difference in each mRNA between S-1 and IRI-S groups, whereas there were differences among some clinical characteristics. Multivariate analyses for overall survival showed that mRNA levels were not correlated with prognosis. By comparison, between IRI-S and S-1 arms, low thymidylate synthase, low ERCC1 and high thymidine phosphorylase were associated with better prognosis for IRI-S versus S-1 (hazard ratio = 0.653, 0.702 and 0.709, respectively; P < 0.15 for each interaction).

Conclusion: Low thymidylate synthase, low ERCC1 and high thymidine phosphorylase are candidates for predictive biomarkers for first-line treatment in advanced gastric cancer by IRI-S. Further study is warranted to confirm these results in other clinical trials and cohort studies.

Keywords: Excision repair cross-complementing gene 1; gastric cancer; irinotecan; predictive factor; thymidine phosphorylase; thymidylate synthase.

Publication types

  • Clinical Trial, Phase III
  • Randomized Controlled Trial

MeSH terms

  • Aged
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
  • Biomarkers, Tumor / analysis*
  • Camptothecin / administration & dosage
  • Camptothecin / analogs & derivatives*
  • DNA Topoisomerases, Type I / analysis
  • DNA-Binding Proteins / analysis
  • Dihydrouracil Dehydrogenase (NADP) / analysis
  • Drug Combinations
  • Endonucleases / analysis
  • Female
  • Humans
  • Irinotecan
  • Male
  • Middle Aged
  • Oxonic Acid / administration & dosage*
  • Prognosis
  • RNA, Messenger / analysis
  • Retrospective Studies
  • Stomach Neoplasms / drug therapy*
  • Tegafur / administration & dosage*
  • Thymidine Phosphorylase / analysis
  • Thymidylate Synthase / analysis

Substances

  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • Drug Combinations
  • RNA, Messenger
  • S 1 (combination)
  • Tegafur
  • Oxonic Acid
  • Irinotecan
  • Dihydrouracil Dehydrogenase (NADP)
  • Thymidylate Synthase
  • Thymidine Phosphorylase
  • ERCC1 protein, human
  • Endonucleases
  • DNA Topoisomerases, Type I
  • TOP1 protein, human
  • Camptothecin