Reactive Infection Control Strategy for Control of New Delhi Metallo-β-Lactamase (NDM)-Producing Enterobacteriaceae Analyzed Using Whole-Genome Sequencing: Hits and Misses

Kalisvar Marimuthu; Oon Tek Ng; Wei Xin Khong; Eryu Xia; Yik-Ying Teo; Rick Twee-Hee Ong; David Chien Lye; Angela Liping Chow; Prabha Krishnan; Brenda Sze Ang

doi:10.1017/ice.2016.92

Reactive Infection Control Strategy for Control of New Delhi Metallo-β-Lactamase (NDM)-Producing Enterobacteriaceae Analyzed Using Whole-Genome Sequencing: Hits and Misses

Infect Control Hosp Epidemiol. 2016 Aug;37(8):987-990. doi: 10.1017/ice.2016.92. Epub 2016 May 4.

Authors

Kalisvar Marimuthu^{1

2}, Oon Tek Ng¹, Wei Xin Khong¹, Eryu Xia³, Yik-Ying Teo^{3

4

5

6

7}, Rick Twee-Hee Ong⁴, David Chien Lye^{1

2}, Angela Liping Chow⁸, Prabha Krishnan⁹, Brenda Sze Ang¹

Affiliations

¹ Department of Infectious Diseases, Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore, Singapore.
² Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
³ NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore.
⁴ Centre for Infectious Disease Epidemiology and Research, Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore.
⁵ Department of Statistics and Applied Probability, National University of Singapore, Singapore, Singapore.
⁶ Life Sciences Institute, National University of Singapore, Singapore, Singapore.
⁷ Genome Institute of Singapore, Singapore, Singapore.
⁸ Department of Clinical Epidemiology, Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore, Singapore.
⁹ Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore.

PMID: 27143092
DOI: 10.1017/ice.2016.92

Abstract

Genetically distinct isolates of New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae were identified from the clinical cultures of 6 patients. Screening of shared-ward contacts identified 2 additional NDM-positive patients. Phylogenetic analysis proved that 1 contact was a direct transmission while the other was unrelated to the index, suggesting hidden routes of transmission. Infect Control Hosp Epidemiol 2016;37:987-990.

MeSH terms

Aged
Aged, 80 and over
Anti-Bacterial Agents / pharmacology
Drug Resistance, Multiple, Bacterial / drug effects
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics*
Enterobacteriaceae / isolation & purification
Female
Hospitals, Teaching
Humans
Infection Control / methods*
Male
Microbial Sensitivity Tests / methods*
Middle Aged
Retrospective Studies
beta-Lactamases / isolation & purification*

Substances

Anti-Bacterial Agents
beta-Lactamases
beta-lactamase NDM-1