It is well known that lysozymes are key proteins to teleosts in the innate immune system and possess high bactericidal properties. In the present study, a c-type lysozyme gene (To-lysC) was cloned from golden pompano, Trachinotus ovatus. The To-lysC cDNA is composed of 743 bp with a 36 bp of 5'-UTR, 432 bp open reading frame (ORF) and 275 bp 3'-UTR, encoding a polypeptide of 144 amino acids (GenBank accession no: KT935522). Phylogenetic analysis revealed that To-lysC showed highest similarity to Perca flavescens lysC. Quantitative real-time PCR (qRT-PCR) analysis showed that To-lysC had relatively high expression level in the head kidney, gill and brain. After Vibrio harveyi infection, transcripts of To-lysC increased and reached its peak at 12 h p.i. These results indicated that To-lysC may play an important role in innate immune response to bacteria.
Keywords: Innate immune; Trachinotusovatus; Vibrio harveyi; c-type lysozyme gene.
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