A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

Placenta. 2016 Aug:44:109-11. doi: 10.1016/j.placenta.2016.04.014. Epub 2016 Apr 30.

Abstract

A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.

Keywords: Free peptide; LC-MS; Multivariate analysis; Placenta; Proteolysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid
  • Delivery, Obstetric
  • Female
  • Humans
  • Mass Spectrometry
  • Placenta / metabolism*
  • Placenta / pathology
  • Pregnancy
  • Proteolysis
  • Proteomics / methods*
  • Specimen Handling