Assembling Multi-subunit Complexes Using Mammalian Expression

Bahar Baser; Joop van den Heuvel

doi:10.1007/978-3-319-27216-0_15

Assembling Multi-subunit Complexes Using Mammalian Expression

Adv Exp Med Biol. 2016:896:225-38. doi: 10.1007/978-3-319-27216-0_15.

Authors

Bahar Baser¹, Joop van den Heuvel²

Affiliations

¹ Department of Structure and Function of Proteins, Helmholtz Centre for Infection Research, Braunschweig, Germany.
² Department of Structure and Function of Proteins, Helmholtz Centre for Infection Research, Braunschweig, Germany. [email protected].

PMID: 27165329
DOI: 10.1007/978-3-319-27216-0_15

Abstract

In this chapter conventional and emerging new technologies for the production of complex biologics in mammalian expression systems are summarized. The essential features of the most relevant methods to generate stable production cell lines for the expression of recombinant multi-protein complexes are described. Especially the promising multiple targeted integration strategy by Flp or CRISPR/Cas9 mediated recombination and their future impact on multi-protein expression are highlighted.

Keywords: Flp/FRT recombination; Multi RMCE; MultiMam; Stable cell line; Targeted integration.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
CRISPR-Cas Systems
Cell Culture Techniques
Cell Line
DNA Nucleotidyltransferases / genetics
DNA Nucleotidyltransferases / metabolism
Gene Expression Regulation
Genetic Vectors
Humans
Multiprotein Complexes
Protein Engineering / methods*
Protein Multimerization
Protein Structure, Quaternary
Protein Subunits
Recombinant Proteins / biosynthesis*
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Structure-Activity Relationship
Transcription, Genetic
Transfection

Substances

Multiprotein Complexes
Protein Subunits
Recombinant Proteins
DNA Nucleotidyltransferases
FLP recombinase