Electroporation of siRNA to Silence Gene Expression in Primary NK Cells

Prasad V Phatarpekar; Dean A Lee; Srinivas S Somanchi

doi:10.1007/978-1-4939-3684-7_22

Electroporation of siRNA to Silence Gene Expression in Primary NK Cells

Methods Mol Biol. 2016:1441:267-76. doi: 10.1007/978-1-4939-3684-7_22.

Authors

Prasad V Phatarpekar¹, Dean A Lee¹, Srinivas S Somanchi²

Affiliations

¹ Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA.
² Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA. [email protected].

PMID: 27177673
DOI: 10.1007/978-1-4939-3684-7_22

Abstract

Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection(®). The technique yields effective silencing of the target gene without any off-target effects.

Keywords: Electroporation; Gene silencing; Human primary NK cell; Nucleofection; ON-TARGETplus; SMART pool siRNA; siRNA.

MeSH terms

Cell Survival
Cells, Cultured
Electroporation / methods*
Gene Expression
Gene Silencing
Humans
Killer Cells, Natural / cytology*
Killer Cells, Natural / metabolism
RNA, Small Interfering / genetics*

Substances

RNA, Small Interfering