A competitive luminol chemiluminescence immunosensor based on a microfluidic chip for the determination of ractopamine

Sai Wang; Qilong Chen; Xiao Wei; Jian Wu; Chunyan Wang; Jiahui Liu; Liya Zhang; Yiyang Dong

doi:10.1002/elps.201600179

A competitive luminol chemiluminescence immunosensor based on a microfluidic chip for the determination of ractopamine

Electrophoresis. 2017 Jan;38(2):368-371. doi: 10.1002/elps.201600179. Epub 2016 Jun 7.

Authors

Sai Wang¹, Qilong Chen¹, Xiao Wei¹, Jian Wu², Chunyan Wang³, Jiahui Liu¹, Liya Zhang¹, Yiyang Dong¹

Affiliations

¹ Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
² Institute of Laser Engineering, Beijing University of Technology, Beijing, P. R. China.
³ State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, P. R. China.

PMID: 27189507
DOI: 10.1002/elps.201600179

Abstract

Herein, a competitive luminol chemiluminescence immunosensor based on a microfluidic chip was developed to detect ractopamine (RCT) both in phosphate buffer and swine urine samples. The immunosensor can provide a liner range of 0.5-40 ng/mL and a high sensitivity with a limit of detection of 0.97 ng/mL for RCT detection in swine urine. Good rates of recovery in negative swine urine samples were achieved over the RCT concentration ranging from 0.5 to 40 ng/mL. The proposed method offered a promising analytical scheme for the on-site determination of RCT.

Keywords: Immunosensor; Luminol chemiluminescence; Microfluidic chip; Ractopamine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Immunoassay / methods*
Luminescent Measurements / methods*
Luminol / analysis
Luminol / chemistry*
Microfluidic Analytical Techniques / methods*
Phenethylamines / urine*
Swine

Substances

Phenethylamines
ractopamine
Luminol