Blocking of urotensin receptors as new target for treatment of carrageenan induced inflammation in rats

Peptides. 2016 Aug:82:35-43. doi: 10.1016/j.peptides.2016.05.006. Epub 2016 May 18.

Abstract

This study investigated possible role of U-II and its receptor expression in inflammation by using UTR agonist and antagonist in carrageenan induced acute inflammation. Rats were divided into 5 groups as (1) Healthy control, (2) Carrageenan control, (3) Carrageenan +Indomethacin 20mg/kg, orally, (4) Carrageenan +AC7954 (U-II receptor agonist, intraperitoneally) 30mg/kg and (5) Carrageenan +SB657510 (UTR antagonist, intraperitoneally) 30mg/kg. 1h after drug administration, carrageenan was injected. At the 3rd hour after carrageenan injection, agonist produced no effect while antagonist 63% anti-inflammatory effect respectively. UTR and UT-II expression increased in carrageenan induced paw tissue. Antagonist administration prevented the decrease in an antioxidant system and also capable to decrease TNF-α and IL-6 mRNA expressions. This study showed the role of urotensin II receptors in the physiopathogenesis of acute inflammatory response that underlying many diseases accompanied by inflammation.

Keywords: Inflammation; Rats; SB657510; TNF-Alpha; Urotensin II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Carrageenan / toxicity
  • Chromans / administration & dosage
  • Gene Expression Regulation / drug effects
  • Humans
  • Indomethacin / administration & dosage
  • Inflammation / chemically induced
  • Inflammation / drug therapy*
  • Inflammation / pathology
  • Interleukin-6 / genetics
  • Rats
  • Receptors, G-Protein-Coupled / agonists
  • Receptors, G-Protein-Coupled / antagonists & inhibitors
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Tumor Necrosis Factor-alpha / genetics
  • Urotensins / genetics
  • Urotensins / metabolism*

Substances

  • 3-(4-chlorophenyl)-3-(2-(dimethylamino)ethyl)isochroman-1-one
  • Anti-Inflammatory Agents
  • Chromans
  • Interleukin-6
  • Receptors, G-Protein-Coupled
  • Tumor Necrosis Factor-alpha
  • Urotensins
  • Uts2r protein, rat
  • Carrageenan
  • urotensin II
  • Indomethacin