Characterization of purinoceptors present on human liver plasma membranes

S Keppens; A Vandekerckhove; H De Wulf

doi:10.1016/0014-5793(89)80448-x

Characterization of purinoceptors present on human liver plasma membranes

FEBS Lett. 1989 May 8;248(1-2):137-40. doi: 10.1016/0014-5793(89)80448-x.

Authors

S Keppens¹, A Vandekerckhove, H De Wulf

Affiliation

¹ Afdeling Biochemie, Katholieke Universiteit Leuven, Belgium.

PMID: 2721671
DOI: 10.1016/0014-5793(89)80448-x

Abstract

Using ATP alpha 35S as radioligand, we have detected the presence of specific purinoceptors on human liver plasma membranes. They are characterized by a Kd value of 0.19 microM and a Bmax of 24 pmol/mg membrane protein. These purinoceptors belong to the P2Y subclass as demonstrated by the high degree of similarity with rat liver purinoceptors, previously shown to be P2Y [(1986) Biochem. J. 240, 367-371] and known to be involved in the control of liver glycogenolysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Animals
Cell Membrane / analysis*
Humans
Liver / analysis
Liver / metabolism*
Rats
Receptors, Purinergic / classification
Receptors, Purinergic / isolation & purification*
Receptors, Purinergic / physiology
Species Specificity

Substances

Receptors, Purinergic
Adenosine Diphosphate
Adenosine Triphosphate