Multiplex PCR assay underreports true bloodstream infections with coagulase-negative staphylococci in hematological patients with febrile neutropenia

Diagn Microbiol Infect Dis. 2016 Aug;85(4):413-5. doi: 10.1016/j.diagmicrobio.2016.04.024. Epub 2016 May 5.

Abstract

SeptiFast multiplex PCR assay was evaluated for detecting true bloodstream infections (BSIs) with coagulase-negative staphylococci (CoNS) in neutropenic hematological patients. Sensitivity for samples representing true CoNS-BSIs was 23.3% with an integrated cutoff and increased to 83.3% if the cutoff was neglected. Hence, the cutoff may prohibit timely targeted antimicrobial therapy.

Keywords: Bloodstream infections; Coagulase-negative staphylococci; Cutoff; Febrile neutropenia; Multiplex PCR.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Bacteriological Techniques / methods
  • Coagulase / analysis*
  • Febrile Neutropenia / diagnosis*
  • Febrile Neutropenia / microbiology
  • Hematologic Neoplasms / complications*
  • Humans
  • Molecular Diagnostic Techniques / methods
  • Multiplex Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sepsis / diagnosis*
  • Sepsis / microbiology
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus / enzymology
  • Staphylococcus / isolation & purification*

Substances

  • Coagulase