Reporter Systems to Study Cancer Stem Cells

Caner Saygin; Mohamed Samour; Anastasia Chumakova; Awad Jarrar; Justin D Lathia; Ofer Reizes

doi:10.1007/7651_2016_360

Reporter Systems to Study Cancer Stem Cells

Methods Mol Biol. 2016:1516:319-333. doi: 10.1007/7651_2016_360.

Authors

Caner Saygin¹, Mohamed Samour^{1

2}, Anastasia Chumakova¹, Awad Jarrar¹, Justin D Lathia^{1

3

4

5}, Ofer Reizes^{6

7

8

9}

Affiliations

¹ Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.
² Medicine Institute, Cleveland Clinic, Cleveland, OH, 44195, USA.
³ Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, 44195, USA.
⁴ Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH, 44195, USA.
⁵ Case Comprehensive Cancer Center, Cleveland, OH, 44106, USA.
⁶ Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA. [email protected].
⁷ Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, 44195, USA. [email protected].
⁸ Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH, 44195, USA. [email protected].
⁹ Case Comprehensive Cancer Center, Cleveland, OH, 44106, USA. [email protected].

PMID: 27221339
DOI: 10.1007/7651_2016_360

Abstract

Cancer stem cells have been identified in primary tumors, patient derived xenografts, and established cancer cell lines. The development of reporters has enabled investigators to rapidly enrich for these cells and more importantly track these cells in real time. Here we describe the current state of the reporter field and their use and limitations in multiple cancers.

Keywords: Cancer stem cells; NANOG; Pluripotency transcription factor; Reporter; Tumor heterogeneity.

MeSH terms

Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism
Genes, Reporter / genetics*
Humans
Molecular Biology / methods*
Nanog Homeobox Protein / genetics
Nanog Homeobox Protein / metabolism
Neoplasms / genetics*
Neoplasms / pathology
Neoplastic Stem Cells / metabolism*
Neoplastic Stem Cells / pathology

Substances

Nanog Homeobox Protein