Effective healing of chronic rotator cuff injury using recombinant bone morphogenetic protein-2 coated dermal patch in vivo

J Biomed Mater Res B Appl Biomater. 2017 Oct;105(7):1840-1846. doi: 10.1002/jbm.b.33716. Epub 2016 May 26.

Abstract

Biologic augmentation for rotator cuff repair is a challenging treatment in patients with chronic large, massive, and irreparable rotator cuff injuries. Particularly, the use of an extracellular matrix (ECM) patch such as dermal tissue offered improved biomechanical properties in previous studies. Cytokines induce cell chemotaxis, proliferation, matrix synthesis, and cell differentiation. Moreover, osteoinductive growth factors such as bone morphogenetic protein-2 (BMP-2) affect the formation of new bone and fibrocartilage in lesions. However, the effects of using a dermal patch in combination with BMP-2 have not been evaluated to date, although many researchers have recognized the importance thereof. In this study, rhBMP-2-coated dermal patch (1 cm × 2 cm) isolated from human cadaveric donor was inserted in a rabbit model of chronic rotator cuff injury for in vivo evaluation. Bone mineral density and biomechanical strength were tested and histological and histomorphometric analyses were performed. The results showed that insertion of an rhBMP-2-coated acellular dermal patch not only significantly ameliorated new bone formation, it also improved biomechanical properties such as ultimate tensile strength. Thus, the use of this combination may improve the chronic rotator cuff injury-healing rate and clinical outcomes after rotator cuff repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1840-1846, 2017.

Keywords: acellular dermal patch; enthesis generation; rhBMP-2; rotator cuff; tendon-bone healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Dressings*
  • Bone Morphogenetic Protein 2* / chemistry
  • Bone Morphogenetic Protein 2* / pharmacokinetics
  • Bone Morphogenetic Protein 2* / pharmacology
  • Chronic Disease
  • Coated Materials, Biocompatible* / chemistry
  • Coated Materials, Biocompatible* / pharmacokinetics
  • Coated Materials, Biocompatible* / pharmacology
  • Male
  • Rabbits
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / pharmacokinetics
  • Recombinant Proteins / pharmacology
  • Rotator Cuff Injuries / therapy*
  • Skin*

Substances

  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Coated Materials, Biocompatible
  • Recombinant Proteins