Investigating Salmonella Eko from Various Sources in Nigeria by Whole Genome Sequencing to Identify the Source of Human Infections

Pimlapas Leekitcharoenphon; Ibrahim Raufu; Mette T Nielsen; Birthe S Rosenqvist Lund; James A Ameh; Abdul G Ambali; Gitte Sørensen; Simon Le Hello; Frank M Aarestrup; Rene S Hendriksen

doi:10.1371/journal.pone.0156212

Investigating Salmonella Eko from Various Sources in Nigeria by Whole Genome Sequencing to Identify the Source of Human Infections

PLoS One. 2016 May 26;11(5):e0156212. doi: 10.1371/journal.pone.0156212. eCollection 2016.

Affiliations

¹ National Food Institute, Technical University of Denmark, WHO Collaborating Center for Antimicrobial Resistance in Foodborne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, Kgs. Lyngby, Denmark.
² Faculty of Veterinary Medicine, Department of Veterinary Microbiology, University of Ilorin, Ilorin, Nigeria.
³ National Food Institute, Technical University of Denmark, Søborg, Denmark.
⁴ Faculty of Veterinary Medicine, Department of Veterinary Microbiology and Parasitology, University of Abuja, Abuja, Nigeria.
⁵ Institut Pasteur, Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Salmonella, Paris, France.

Abstract

Twenty-six Salmonella enterica serovar Eko isolated from various sources in Nigeria were investigated by whole genome sequencing to identify the source of human infections. Diversity among the isolates was observed and camel and cattle were identified as the primary reservoirs and the most likely source of the human infections.

MeSH terms

Animals
Camelus
Cattle
DNA, Bacterial / genetics*
Fishes
Food Microbiology*
Genetic Variation / genetics
Genome, Bacterial*
High-Throughput Nucleotide Sequencing / methods*
Humans
Phylogeny
Salmonella Infections / microbiology*
Salmonella enterica / classification
Salmonella enterica / genetics*
Salmonella enterica / isolation & purification*
Salmonella enterica / pathogenicity
Virulence / genetics

Substances

DNA, Bacterial

Grants and funding

This work was supported by the Danish Council for Strategic Research (grant number: 09-067103), Center for Genomic Epidemiology (www.genomicepidemiology.org), and by the World Health Organization Global Foodborne Infections Network (www.who.int/gfn).