Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit

Chow Wenn Yew; Yee Joo Tan

doi:10.1007/978-1-4939-3618-2_20

Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit

Methods Mol Biol. 2016:1426:225-33. doi: 10.1007/978-1-4939-3618-2_20.

Authors

Chow Wenn Yew¹, Yee Joo Tan^{2

3}

Affiliations

¹ Monoclonal Antibody Unit, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, 138673, Singapore.
² Monoclonal Antibody Unit, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, 138673, Singapore. [email protected].
³ Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, 5 Science Drive 2, Blk MD4, Level 3, Singapore, 117545, Singapore. [email protected].

PMID: 27233275
DOI: 10.1007/978-1-4939-3618-2_20

Abstract

Monoclonal antibodies offer high specificity and this makes it an important tool for molecular biology, biochemistry and medicine. Typically, monoclonal antibodies are generated by fusing mouse spleen cells that have been immunized with the desired antigen with myeloma cells to create immortalized hybridomas. Here, we describe the generation of monoclonal antibodies that are specific to Chikungunya virus using ClonaCell-HY system.

Keywords: Chikungunya virus; Fusion; Immunofluorescence; Monoclonal antibody; Screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / metabolism*
Antigens, Viral / immunology*
Cell Line
Chikungunya virus / immunology*
Immunization
Mice
Spleen / cytology
Spleen / immunology

Substances

Antibodies, Monoclonal
Antigens, Viral