Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo

Zina Jawadi; Brian E Applegate; John S Oghalai

doi:10.1007/978-1-4939-3615-1_24

Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo

Methods Mol Biol. 2016:1427:449-62. doi: 10.1007/978-1-4939-3615-1_24.

Authors

Zina Jawadi¹, Brian E Applegate², John S Oghalai³

Affiliations

¹ Department of Otolaryngology-Head and Neck Surgery, Stanford University, 300 Pasteur Dr., Edwards R113, Stanford, CA, 94305, USA.
² Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technology Building, College Station, TX, 77843, USA.
³ Department of Otolaryngology-Head and Neck Surgery, Stanford University, 300 Pasteur Dr., Edwards R113, Stanford, CA, 94305, USA. [email protected].

Abstract

The measurement of mechanical vibrations within the living cochlea is critical to understanding the first nonlinear steps in auditory processing, hair cell stimulation, and cochlear amplification. However, it has proven to be a challenging endeavor. This chapter describes how optical coherence tomography (OCT) can be used to measure vibrations within the tissues of the organ of Corti. These experimental measurements can be performed within the unopened cochlea of living mice routinely and reliably.

Keywords: Basilar membrane; Biomechanics; Cochlear mechanics; In vivo imaging; Sound encoding; Tectorial membrane; Vibrometry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Acoustic Stimulation
Animals
Basilar Membrane / physiology
Biomechanical Phenomena
Mice
Organ of Corti / physiology*
Sound
Tectorial Membrane / physiology
Tomography, Optical Coherence / instrumentation*
Tomography, Optical Coherence / methods
Vibration

Abstract

Publication types

MeSH terms

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