Ultrasensitive detection of inhaled organic aerosol particles by accelerator mass spectrometry

E V Parkhomchuk; D G Gulevich; A I Taratayko; A M Baklanov; A V Selivanova; T A Trubitsyna; I V Voronova; P N Kalinkin; A G Okunev; S A Rastigeev; V A Reznikov; V S Semeykina; K A Sashkina; V V Parkhomchuk

doi:10.1016/j.chemosphere.2016.05.078

Ultrasensitive detection of inhaled organic aerosol particles by accelerator mass spectrometry

Chemosphere. 2016 Sep:159:80-88. doi: 10.1016/j.chemosphere.2016.05.078. Epub 2016 Jun 6.

Authors

Affiliations

¹ Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia. Electronic address: [email protected].
² Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
³ Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentieva st., Novosibirsk, 630090, Russia.
⁴ Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya st., Novosibirsk, 630090, Russia.
⁵ Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; JSC Tion, 20 Injenernaya str., 630090, Novosibirsk, Russia.
⁶ JSC Tion, 20 Injenernaya str., 630090, Novosibirsk, Russia.
⁷ Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia.
⁸ Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Budker Institute of Nuclear Physics SB RAS, 11 Lavrentieva st., Novosibirsk 630090, Russia.

PMID: 27281540
DOI: 10.1016/j.chemosphere.2016.05.078

Abstract

Accelerator mass spectrometry (AMS) was shown to be applicable for studying the penetration of organic aerosols, inhaled by laboratory mice at ultra-low concentration ca. 10(3) cm(-3). We synthesized polystyrene (PS) beads, composed of radiocarbon-labeled styrene, for testing them as model organic aerosols. As a source of radiocarbon we used methyl alcohol with radioactivity. Radiolabeled polystyrene beads were obtained by emulsifier-free emulsion polymerization of synthesized (14)C-styrene initiated by K2S2O8 in aqueous media. Aerosol particles were produced by pneumatic spraying of diluted (14)C-PS latex. Mice inhaled (14)C-PS aerosol consisting of the mix of 10(3) 225-nm particles per 1 cm(3) and 5·10(3) 25-nm particles per 1 cm(3) for 30 min every day during five days. Several millions of 225-nm particles deposited in the lungs and slowly excreted from them during two weeks of postexposure. Penetration of particles matter was also observed for liver, kidneys and brain, but not for a heart.

Keywords: Accelerator mass-spectrometry; Low-dose inhalation; Mice; Organic aerosols; Polystyrene beads; Radiocarbon.

MeSH terms

Administration, Inhalation
Aerosols
Animals
Brain / metabolism
Carbon Radioisotopes
Kidney / metabolism
Liver / metabolism
Lung / metabolism
Male
Mass Spectrometry / methods
Mice, Inbred CBA
Particle Size
Polystyrenes / administration & dosage
Polystyrenes / analysis*
Polystyrenes / pharmacokinetics

Substances

Aerosols
Carbon Radioisotopes
Polystyrenes