RTFBSDB: an integrated framework for transcription factor binding site analysis

Bioinformatics. 2016 Oct 1;32(19):3024-6. doi: 10.1093/bioinformatics/btw338. Epub 2016 Jun 10.

Abstract

Transcription factors (TFs) regulate complex programs of gene transcription by binding to short DNA sequence motifs. Here, we introduce rtfbsdb, a unified framework that integrates a database of more than 65 000 TF binding motifs with tools to easily and efficiently scan target genome sequences. Rtfbsdb clusters motifs with similar DNA sequence specificities and integrates RNA-seq or PRO-seq data to restrict analyses to motifs recognized by TFs expressed in the cell type of interest. Our package allows common analyses to be performed rapidly in an integrated environment.

Availability and implementation: rtfbsdb available at (https://github.com/Danko-Lab/rtfbs_db).

Contact: [email protected]

Supplementary information: Supplementary data are available at Bioinformatics online.

MeSH terms

  • Animals
  • Binding Sites*
  • Computational Biology
  • Genome
  • Humans
  • Nucleotide Motifs
  • Protein Binding
  • Transcription Factors* / chemistry

Substances

  • Transcription Factors